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laboratory tool

Reconstitution calculator

Vial amount, solvent volume, amount to measure: the volume to draw and the syringe mark appear instantly, with the conversion table for your solution.

1The vial
2The solvent
mL

The volume set by your method or the batch document. Solvent: bacteriostatic water 3 mL or 10 mL.

3The amount to measure

The value set by your experimental protocol. 1 mg = 1,000 µg: the calculator converts for you.

4The syringe

U-100 syringes: 100 units = 1 mL, whatever their size. 1 mL syringes, box of 100.

A prefilled pen is already in solution: it is not reconstituted. See the pens

Your solution

Conversion table

What each syringe mark contains, for the solution calculated above. Tap a row to use it as the amount to measure.

UnitsVolumeQuantity

The calculation, step by step

  1. 1

    Concentration

    Amount in the vial ÷ solvent volume.

    10 mg ÷ 2 mL = 5 mg/mL

  2. 2

    Volume to draw

    Amount to measure ÷ concentration.

    0,5 mg ÷ 5 mg/mL = 0,1 mL

  3. 3

    Mark on the syringe

    Volume × 100 on a U-100 syringe.

    0,1 mL × 100 = 10

Good to know

  • U-100 refers to the scale, not the size: on a 0.3 mL, 0.5 mL or 1 mL syringe, 10 units always equal 0.1 mL.
  • Less solvent gives a more concentrated solution: fewer units for the same amount, but a less precise reading.
  • Hormones measured in international units (HGH, FSH, HCG) are calculated in IU: the concentration is then expressed in IU per mL.
  • For a blend, the concentration refers to the total mass; the calculator also shows each component.

Frequently asked questions

How much solvent should be added?

The amount set by your method or the batch document. The calculator does not recommend one: it shows the effect of each volume on the concentration and on the syringe reading.

Why does the reading fall between two marks?

The requested amount does not match a whole mark. The calculator shows the closest mark and, where possible, a solvent volume that puts the reading exactly on a mark.

Are my values saved?

Your latest values stay in this browser, on this device only. Nothing is sent to PeptidLab. The Clear button deletes them.

Conversion tool intended for laboratory research only. It does not recommend any solvent, concentration or quantity to use. Verify solvent compatibility, instrument capacity and each data with your method and batch document before handling.